Sarcoma cancer bone, Bone Cancer, Primary Bone Cancers and Bone Metastases, Sarcoma cancer of the bone


Limb Salvage Surgery for Bone Sarcoma - Ben Miller, MD

Source: Fiziologia - Physiology. Abstract: Background: The adult mesenchymal stem cells MSCs are one of the most studied populations of adult multipotent stem cells, being isolated from bone marrow, peripheral blood, or other solid tissues. The fetal MSCs are isolated from placenta, amniotic fluid, umbilical cord and umbilical cord blood. Although they do not have the same proliferative and differentiation potentials of ESCs, the sarcoma cancer bone multipotent cells may be superior to adult stem cells, so that their increased potential should be explored in more detail for further therapeutic strategies.

Materials and methods: Placental stem cells PSCs were isolated from 20 term-delivered placentas using sarcoma cancer bone digestion Collagenase I A.

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Morphological characterization showed fibroblast-shape cellular appearance, adherent to plastic surface, and reaching confluence in spindle-like colonies. Tumor formation was completed in 4 weeks. For comparative analysis we used bone marrow-derived MSCs stably expressing Sarcoma cancer bone, and we visualized cellular migration, homing and effects by serial pictures taken every 3 days for 2 weeks using in vivo imaging system Hamamatsu Aequoria.

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Results: PSCs obtained using enzymatic digestion were similar to bone marrow-derived mesenchymal stem cells in morphological and functional characteristics. Conclusion: The present study shows that PSCs have an increased ability to modulate tumor growth compared to their counterparts, the bone marrow-derived MSCs, which makes them more suitable for therapeutic approaches in cancer treatment.

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